Debora Esposito, PhD MBS
Director, Cell Culture and Phenotyping Core
Tel: +1 (704) 250-5465
Fax: +1 (704) 250-5425
Email: daesposi at ncsu.edu
Plants for Human Health Institute at the NC State University houses Cell Culture and Phenotyping Core dedicated to the assessment of gene-nutrient interactions, metabolic fluxes, hormone levels, and signaling pathways in cell culture, animal, and human research studies.
The Core’s assay services are available to researchers from within the NC State University and throughout the world. Researchers may send samples directly to the core to be assayed on a service rate basis (see below). In addition to assay services, the Core also serves as a training facility for faculty and students who are interested in including measures of metabolic, immune, and skin system functioning in their research. We anticipate offering training sessions each year with Core Director and the laboratory technician.
To submit a service request by email, please include the following information: PI name, address, contact information, assays to be performed, number of samples, and how the samples were collected and processed.
The Biosafety Level 2 suite is equipped with a laminar-flow hood, CO2 incubator, Bellco Roll-In Incubator, Zeiss Axio Observer inverted microscope, EVOS FL Auto Imaging System with onstage incubator, Life StepOnePlus Real-Time PCR thermal cycler, Orflo Moxi automated cell counter, multiple refrigerators (4 °C), freezers (-20 °C), ulta-low freezers (-80 °C), and a liquid nitrogen freezer (-190 °C) for long-term storage and cryopreservation.
The main lab is equipped with ABI Gene Amp 9700 PCR and 7500 Fast Real-Time PCR thermal cyclers, BioTek Synergy H1 microplate reader (absorbance, fluorescence, and luminescence), XF-24 Seahorse Extracellular Flux Analyzer, BD Accuri C6 Flow Cytometer, Sorvall RC6 Plus and Legend T Plus centrifuges, and multiple instruments for routine nucleic acid and protein analysis, including Western blot and ELISA.
Please register a free account at Quartzy for core/equipment schedules and sign-ups.
If you are certain you would like to proceed with using the Core services, please submit Core service request by email. Please include the following information: PI name, address, contact information, assays to be performed, number of samples, and how the samples were collected and processed.
In response to this email, the Core director will provide information on: getting your samples to Core, schedule availability, pricing, and any special instructions on processing.
Once you contact Core, we’ll set up a time schedule for when you can expect your sample results. All samples are run at least in duplicate. The standards and quality control in the Core are extremely high, and you can expect that your results will be precise and accurate. Additionally, while your samples are in the Core, you can expect that they will be stored and handled with the utmost care at whichever temperature you choose. Once your samples have been successfully assayed, we can either dispose of your samples or return them to you.
We can provide you with descriptions of space and equipment, review the assay methods section of your grant, and suggest a budget estimate for the proposed work. Please contact the Core director by email and provide the relevant details of your proposal.
If you are willing to share the appropriate cell culture and any non-standard reagents and/or growth factors required for its maintenance, Core will seek a biological safety approval for a new cell line prior to performing the requested assay at no additional cost.
Please note that all researchers must have already secured their own IACUC approval if working with animals or IRB approval if working with humans.